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rabbit polyclonal anticofilin antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anticofilin antibody
    nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and <t>anticofilin</t> and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].
    Rabbit Polyclonal Anticofilin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anticofilin+antibody/anti+phospho+cofilin/pmc04914163-338-0-9
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anticofilin antibody - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "nArgBP2 regulates excitatory synapse formation by controlling dendritic spine morphology"

    Article Title: nArgBP2 regulates excitatory synapse formation by controlling dendritic spine morphology

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1600944113

    nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and anticofilin and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].
    Figure Legend Snippet: nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and anticofilin and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].

    Techniques Used: Infection, shRNA, Western Blot, Expressing, Construct, Transfection, Mutagenesis



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    rabbit polyclonal anticofilin antibody  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc rabbit polyclonal anticofilin antibody
    nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and <t>anticofilin</t> and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].
    Rabbit Polyclonal Anticofilin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anticofilin+antibody/anti+phospho+cofilin/pmc04914163-338-0-9
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anticofilin antibody - by Bioz Stars, 2026-09
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    polyclonal rabbit anticofilin antibody  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc polyclonal rabbit anticofilin antibody
    nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and <t>anticofilin</t> and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].
    Polyclonal Rabbit Anticofilin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    polyclonal rabbit anticofilin antibody - by Bioz Stars, 2026-09
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    anticofilin 2 rabbit polyclonal antibody (upstate)  (Millipore)
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    Millipore anticofilin 2 rabbit polyclonal antibody (upstate)
    nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and <t>anticofilin</t> and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].
    Anticofilin 2 Rabbit Polyclonal Antibody (Upstate), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anticofilin+antibody/anticofilin+2+rabbit+polyclonal+antibody++upstate+/pm20503409-56-56-72
    Average 90 stars, based on 1 article reviews
    anticofilin 2 rabbit polyclonal antibody (upstate) - by Bioz Stars, 2026-09
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    Image Search Results


    nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and anticofilin and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: nArgBP2 regulates excitatory synapse formation by controlling dendritic spine morphology

    doi: 10.1073/pnas.1600944113

    Figure Lengend Snippet: nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and anticofilin and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].

    Article Snippet: Rabbit polyclonal anticofilin antibody and phosphocofilin antibody were from Cell Signaling Technology.

    Techniques: Infection, shRNA, Western Blot, Expressing, Construct, Transfection, Mutagenesis